Kho was born and raised in Java to a poor family in an eggplant farm where his father, Han Ting was the head of the village. He had three brothers and three sisters. After he had been dropped out of school he started playing tennis at the age of 14, while working in a sports equipment store. In the early years he had troubles to make his father understand his admiration for the game. In 1929 he won the Central Java Tennis Championship. In 1932 his parents passed away. He won the All-Java Championship in 1933. In 1933 he won the Chinese national championships topping Qiufei Hai of Shanghai. In 1935 he became the Chinese national champion for the second time after beating compatriot Khoo Hooi Hye and subsequently was named the top Chinese player. Local people raised money for him to support his post-graduate education in Switzerland. In 1936 he arrived to Great Britain to attend college and in the mean time he was offered a job at the Chinese Embassy. He studied commerce in London. Later he was sponsored by the Dunlop Rubber Company.
He played his first international matches in 1934 when he toured Sumatra challenging the Netherlands Davis Cup team. In Kisaran he met Cornelius Bryan (Champion of Sumatra) whom he beat after e decider third set. In doubles Bryan and team member Henrik Timmer equalized the tally in a three-set victory against the Chinese. Then they moved to Siantar where at the inaugural of the Simeloengoen Club two one-set matches were held. Bryan and Kho went toe-to-toe in singles with Kho victorious. Then he faced Alsbach and won six to four. This was followed by the doubles between Timmer-Bryan and Alsbach-Kho, which was abandoned due to Bryan's injury with Khos' leading one set and one break in the second, while the Bryan was serving to stay in the match. In Medan he beat Bryan in a normal match in straights and had his match with Alsbach interrupted as it started raining.
In 1935 the Dutch Indies Tennis Association invited a couple of European players to play a series of exhibition matches in the Orient. In Surabaya he paired with Nami to face Giorgio de Stefani and Enrique Maier and lost in two sets. Then the party traveled to Surakarta where singles matches were held and Kho faced de Stefani with the Italian coming out victoriously. Then on a rematch in Semarang Kho prevailed fro the first time over de Stefani in front a home crowd of two thousand. While touring America Kho reached the doubles finals of the 1935 Kansas City Championships alongside Lewis D Carson eventually ceding the victory to Wilbur Coen and William Kiley. The same year he debuted in the Davis Cup against the United States Davis Cup team and lost all three of his rubbers.
In 1945 he won the Midlands Counties Championships for the second time and retained his title the next year against Argentine Enrique Morea. In 1946 he went for the North of England title but was eliminated in both the singles and doubles finals by Jack Edwin Harper and Harper-Cam Malfroy respectively. In May he served the first and only Davis Cup victory for the First Republic of China by winning all three of his rubbers against the Denmark Davis Cup team. While in London on 31 January 1947, Kho was admitted to hospital with double pneumonia and died soon after.
Kho appeared in six Davis Cup ties for China, between 1935â1946. He won eight of his 18 rubbers.
In the club level scene he represented the International Tennis Club of Great Britain.
On January 27, 1940 he married Jane Margaret Gordon Balfour, daughter of E.J. Gordon Balfour, a judge in Ceylon. They've met in England at the Queen's Club where both of them practised. They moved to the Dutch East Indies and stayed there for the time of the war. In order to help his country against the Empire of Japan he participated in a series of exhibition matches with Englishman Pat Hughes in the Malacca Lawn Tennis Club. The ticket sales income was transferred to the Malaya Patriotic Fund and the War Fund. In 1940 he became Malayan Champion in singles and mixed doubles. He continued on to get featured in exhibition matches in 1941.
In an 1938 article contemporary Australian tennis player and subsequent chairman of Lawn Tennis Association of Victoria Mervyn Weston described Kho as a drop shot expert, who has a fine control over the ball disguising it so well that it reached a "deadly effect". He had a whipped forehand accompanied by an excellent net game and service and assisted by a formidable backhand. He possessed an athletic body type with a height of 5'11". He was a calm but opportunist player.